Wachten laboratory
Optogenetics
Tool development
Project details
We develop optogenetic tools and genetically encoded biosensors to manipulate and analyze cellular signaling with a focus on subcellular compartments. In addition, we are aiming to transfer tools that allow to control gene expression by light to immune cells in vitro and in vivo.
Project-related publications
Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
Elife. 2020 Jun 24;9:e57907. doi: 10.7554/eLife.57907.
Revisiting and Redesigning Light-Activated Cyclic-Mononucleotide Phosphodiesterases
J Mol Biol. 2019 Aug 9;431(17):3029-3045. doi: 10.1016/j.jmb.2019.07.011. Epub 2019 Jul 10.
Shedding light on the role of cAMP in mammalian sperm physiology
Mol Cell Endocrinol. 2018 Jun 15;468:111-120. doi: 10.1016/j.mce.2017.11.008. Epub 2017 Nov 13.
A novel biosensor to study cAMP dynamics in cilia and flagella
Elife. 2016 Mar 22;5:e14052. doi: 10.7554/eLife.14052.
Controlling fertilization and cAMP signaling in sperm by optogenetics
Elife. 2015 Jan 20;4:e05161. doi: 10.7554/eLife.05161.
Project-related funding
SPP 1926
Deutsche Forschungsgemeinschaft (DFG)
FOR 2743
Deutsche Forschungsgemeinschaft (DFG)
Excellence Cluster ImmunoSensation2
Deutsche Forschungsgemeinschaft (DFG)
SPP 1726
DFG
Project-related scientists
Birthe Stüven
Dagmar Wachten Lab
Jan Niklas Hansen
Dagmar Wachten Lab
Jens-Henning Krause
Dagmar Wachten Lab
Philipp Leyendecker
Dagmar Wachten Lab
Lilian Fu
Dagmar Wachten Lab
Sandro Sieger
