Wachten Laboratory

Cilia

Cilia - a sensory organelle
ScienceTeamPublications

Project details

We study the physiological function of primary cilia in a cell- and tissue-specific context. We are trying to address the main question: How do cilia function as a cellular antenna that receives information from the environment and locally transduces this information into a cellular response?

Ciliary dysfunction results in severe diseases commonly referred as ciliopathies. We aim to address the question why ciliary dysfunction leads to different phenotypes in different tissues. To this end, we work with mouse models as well as human cells and tissues.

Project-related publications

Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium

Hansen JN, Kaiser F, Klausen C, Stüven B, Chong R, Bönigk W, Mick DU, Möglich A, Jurisch-Yaksi N, Schmidt FI, Wachten D.

Elife. 2020 Jun 24;9:e57907. doi: 10.7554/eLife.57907.

Molecular Mechanism Underlying the Action of Zona-pellucida Glycoproteins on Mouse Sperm

Balbach M, Hamzeh H, Jikeli JF, Brenker C, Schiffer C, Hansen JN, Neugebauer P, Trötschel C, Jovine L, Han L, Florman HM, Kaupp UB, Strünker T, Wachten D.

Front Cell Dev Biol. 2020 Aug 31;8:572735. doi: 10.3389/fcell.2020.572735. eCollection 2020.

Rotational motion and rheotaxis of human sperm do not require functional CatSper channels and transmembrane Ca(2+) signaling

Schiffer C, Rieger S, Brenker C, Young S, Hamzeh H, Wachten D, Tüttelmann F, Röpke A, Kaupp UB, Wang T, Wagner A, Krallmann C, Kliesch S, Fallnich C, Strünker T.

EMBO J. 2020 Feb 17;39(4):e102363. doi: 10.15252/embj.2019102363. Epub 2020 Jan 19.

Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models

Guo J, Otis JM, Suciu SK, Catalano C, Xing L, Constable S, Wachten D, Gupton S, Lee J, Lee A, Blackley KH, Ptacek T, Simon JM, Schurmans S, Stuber GD, Caspary T, Anton ES.

Dev Cell. 2019 Dec 16;51(6):759-774.e5. doi: 10.1016/j.devcel.2019.11.005.

Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains

Raju DN, Hansen JN, Rassmann S, Stüven B, Jikeli JF, Strünker T, Körschen HG, Möglich A, Wachten D.

Cells. 2019 Jun 27;8(7):648. doi: 10.3390/cells8070648.

Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development

Olstad EW, Ringers C, Hansen JN, Wens A, Brandt C, Wachten D, Yaksi E, Jurisch-Yaksi N.

Curr Biol. 2019 Jan 21;29(2):229-241.e6. doi: 10.1016/j.cub.2018.11.059. Epub 2019 Jan 3.

A novel biosensor to study cAMP dynamics in cilia and flagella

Mukherjee S, Jansen V, Jikeli JF, Hamzeh H, Alvarez L, Dombrowski M, Balbach M, Strünker T, Seifert R, Kaupp UB, Wachten D.

Elife. 2016 Mar 22;5:e14052. doi: 10.7554/eLife.14052.

Controlling fertilization and cAMP signaling in sperm by optogenetics

Jansen V, Alvarez L, Balbach M, Strünker T, Hegemann P, Kaupp UB, Wachten D.

Elife. 2015 Jan 20;4:e05161. doi: 10.7554/eLife.05161.

Direct action of endocrine disrupting chemicals on human sperm

Schiffer C, Müller A, Egeberg DL, Alvarez L, Brenker C, Rehfeld A, Frederiksen H, Wäschle B, Kaupp UB, Balbach M, Wachten D, Skakkebaek NE, Almstrup K, Strünker T.

EMBO Rep. 2014 Jul;15(7):758-65. doi: 10.15252/embr.201438869. Epub 2014 May 12.

A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation

Hansen JN, Kaiser F, Leyendecker P, Stüven B, Krause JH, Derakhshandeh F, Irfan J, Sroka TJ, Preval KM, Desai PB, Kraut M, Theis H, Drews AD, De-Domenico E, Händler K, Pazour GJ, Henderson DJP, Mick DU, Wachten D

EMBO Rep. 2022 Aug 3;23(8):e54315

Primary cilia and their effects on immune cell functions and metabolism: a model

Ernesto Picon-Galindo, Eicke Latz, Dagmar Wachten,

Trends Immunol. 2022 May;43(5):366-378

Soluble adenylyl cyclase inhibition prevents human sperm functions essential for fertilization

Balbach M, Ghanem L, Rossetti T, Kaur N, Ritagliati C, Ferreira J, Krapf D, Molina LCP, Santi CM, Hansen JN, Wachten D, Fushimi M, Meinke PT, Buck J, Levin LR

Mol Hum Reprod . 2021 Aug 31;gaab054. doi: 10.1093/molehr/gaab054.

Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.

D'Gama PP, Qiu T, Cosacak MI, Rayamajhi D, Konac A, Hansen JN, Ringers C, Acuña-Hinrichsen F, Hui SP, Olstad EW, Chong YL, Lim CKA, Gupta A, Ng CP, Nilges BS, Kashikar ND, Wachten D, Liebl D, Kikuchi K, Kizil C, Yaksi E, Roy S, Jurisch-Yaksi N

Cell Rep. 2021 Oct 5;37(1):109775

Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia function

Haward F, Maslon MM, Yeyati PL, Bellora N, Hansen JN, Aitken S, Lawson J, von Kriegsheim A, Wachten D, Mill P, Adams IR, Caceres JF

Elife. 2021 Aug 2;10:e65104. doi: 10.7554/eLife.65104

Signal transduction in primary cilia - analyzing and manipulating GPCR and second messenger signaling

Wachten D, Mick DU.

Pharmacol Ther. 2021 Aug;224:107836. doi: 10.1016/j.pharmthera.2021.107836

The BBSome: a nexus controlling energy metabolism in the brain

Blaess S, Wachten D.

J Clin Invest. 2021 Apr 15;131(8):148903. doi: 10.1172/JCI148903.

Cfap97d1 is important for flagellar axoneme maintenance and male mouse fertility

Oura S, Kazi S, Savolainen A, Nozawa K, Castañeda J, Yu Z, Miyata H, Matzuk RM, Hansen JN, Wachten D, Matzuk MM, Prunskaite-Hyyryläinen R.

PLoS Genet. 2020 Aug 12;16(8):e1008954. doi: 10.1371/journal.pgen.1008954. eCollection 2020 Aug.

Molecular Mechanism Underlying the Action of Zona-pellucida Glycoproteins on Mouse Sperm

Balbach Melanie, Hamzeh Hussein, Jikeli Jan F., Brenker Christoph, Schiffer Christian, Hansen Jan N., Neugebauer Pia, Trötschel Christian, Jovine Luca, Han Ling, Florman Harvey M., Kaupp U. Benjamin, Strünker Timo, Wachten Dagmar

Front. Cell Dev. Biol., 31 August 2020

Project-related funding

SPP 1926

Deutsche Forschungsgemeinschaft (DFG)

Intramural Funding from the Medical Faculty

Universitätsklinikum Bonn

Boehringer Ingelheim Fonds

Boehringer Ingelheim Fonds

SFB 1454

Deutsche Forschungsgemeinschaft (DFG)

Else Kröner-Fresenius-Stiftung

Else Kröner-Fresenius-Stiftung

Project-related scientists

Christina Klausen, PhD

Administrative Officer

Dagmar Wachten Lab

Fabian Frechen

PhD Student

Dagmar Wachten Lab

Rudolfo Karl

PhD Student

Dagmar Wachten Lab

Arsila Ashraf Palliyulla Kariat

Postdoc

Dagmar Wachten Lab

Birthe Stüven

Alumnus

Dagmar Wachten Lab

Fabian Kaiser

Alumnus

Dagmar Wachten Lab

Jan Niklas Hansen

Alumnus

Dagmar Wachten Lab

Other Projects

Cell-cell communication
Optogenetics
Image analysis
Lipid-protein interactions

Institute of Innate Immunity
University Hospital Bonn
Biomedical Center (BMZ 2)
Building 12
2G
Venusberg-Campus 1
53127 Bonn, Germany

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