Wachten Laboratory

Cilia

Cilia - a sensory organelle

Project details

We study the physiological function of primary cilia in a cell- and tissue-specific context. We are trying to address the main question: How do cilia function as a cellular antenna that receives information from the environment and locally transduces this information into a cellular response?

Ciliary dysfunction results in severe diseases commonly referred as ciliopathies. We aim to address the question why ciliary dysfunction leads to different phenotypes in different tissues. To this end, we work with mouse models as well as human cells and tissues.

Project-related publications

Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium

Hansen JN, Kaiser F, Klausen C, Stüven B, Chong R, Bönigk W, Mick DU, Möglich A, Jurisch-Yaksi N, Schmidt FI, Wachten D.

Elife. 2020 Jun 24;9:e57907. doi: 10.7554/eLife.57907.

Molecular Mechanism Underlying the Action of Zona-pellucida Glycoproteins on Mouse Sperm

Balbach M, Hamzeh H, Jikeli JF, Brenker C, Schiffer C, Hansen JN, Neugebauer P, Trötschel C, Jovine L, Han L, Florman HM, Kaupp UB, Strünker T, Wachten D.

Front Cell Dev Biol. 2020 Aug 31;8:572735. doi: 10.3389/fcell.2020.572735. eCollection 2020.

Rotational motion and rheotaxis of human sperm do not require functional CatSper channels and transmembrane Ca(2+) signaling

Schiffer C, Rieger S, Brenker C, Young S, Hamzeh H, Wachten D, Tüttelmann F, Röpke A, Kaupp UB, Wang T, Wagner A, Krallmann C, Kliesch S, Fallnich C, Strünker T.

EMBO J. 2020 Feb 17;39(4):e102363. doi: 10.15252/embj.2019102363. Epub 2020 Jan 19.

Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models

Guo J, Otis JM, Suciu SK, Catalano C, Xing L, Constable S, Wachten D, Gupton S, Lee J, Lee A, Blackley KH, Ptacek T, Simon JM, Schurmans S, Stuber GD, Caspary T, Anton ES.

Dev Cell. 2019 Dec 16;51(6):759-774.e5. doi: 10.1016/j.devcel.2019.11.005.

Cyclic Nucleotide-Specific Optogenetics Highlights Compartmentalization of the Sperm Flagellum into cAMP Microdomains

Raju DN, Hansen JN, Rassmann S, Stüven B, Jikeli JF, Strünker T, Körschen HG, Möglich A, Wachten D.

Cells. 2019 Jun 27;8(7):648. doi: 10.3390/cells8070648.

Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development

Olstad EW, Ringers C, Hansen JN, Wens A, Brandt C, Wachten D, Yaksi E, Jurisch-Yaksi N.

Curr Biol. 2019 Jan 21;29(2):229-241.e6. doi: 10.1016/j.cub.2018.11.059. Epub 2019 Jan 3.

A novel biosensor to study cAMP dynamics in cilia and flagella

Mukherjee S, Jansen V, Jikeli JF, Hamzeh H, Alvarez L, Dombrowski M, Balbach M, Strünker T, Seifert R, Kaupp UB, Wachten D.

Elife. 2016 Mar 22;5:e14052. doi: 10.7554/eLife.14052.

Controlling fertilization and cAMP signaling in sperm by optogenetics

Jansen V, Alvarez L, Balbach M, Strünker T, Hegemann P, Kaupp UB, Wachten D.

Elife. 2015 Jan 20;4:e05161. doi: 10.7554/eLife.05161.

Direct action of endocrine disrupting chemicals on human sperm

Schiffer C, Müller A, Egeberg DL, Alvarez L, Brenker C, Rehfeld A, Frederiksen H, Wäschle B, Kaupp UB, Balbach M, Wachten D, Skakkebaek NE, Almstrup K, Strünker T.

EMBO Rep. 2014 Jul;15(7):758-65. doi: 10.15252/embr.201438869. Epub 2014 May 12.

Project-related funding

SPP 1926

Deutsche Forschungsgemeinschaft (DFG)

Intramural Funding from the Medical Faculty

Universitätsklinikum Bonn

Boehringer Ingelheim Fonds

Boehringer Ingelheim Fonds

Project-related scientists

Dagmar Wachten Lab
Dagmar Wachten Lab
Dagmar Wachten Lab
Dagmar Wachten Lab

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